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(A) Schematic workflow of alkaloid library screening for anticancer candidates. (B) The CCK-8 assay indicated the inhibitory effects of 269 alkaloid compounds on HCC cell viability. The top three candidate alkaloids with obvious anti-HCC activity were also displayed. (C-D) The time- and dose-dependent effects of PAL on HCC cells Huh7 and HepG2. (E) Changes in the viability of normal hepatic HHL5 cells after PAL treatment. (F) Clonogenic survival assays indicated the roles of PAL in regulating cell growth. <t>(G)</t> <t>Live/dead</t> cell staining indicated the roles of PAL in regulating cell death. Calcein AM (green) stained live cells, and PI (red) labeled dead cells. NS, not significant. * P < 0.05; ** P < 0.01. HCC, hepatocellular carcinoma; CCK-8, Cell Counting Kit-8; PAL, palmatine; PI, propidium iodide; AM, acetoxymethyl ester; DMSO, dimethyl sulfoxide.
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(A) Schematic workflow of alkaloid library screening for anticancer candidates. (B) The CCK-8 assay indicated the inhibitory effects of 269 alkaloid compounds on HCC cell viability. The top three candidate alkaloids with obvious anti-HCC activity were also displayed. (C-D) The time- and dose-dependent effects of PAL on HCC cells Huh7 and HepG2. (E) Changes in the viability of normal hepatic HHL5 cells after PAL treatment. (F) Clonogenic survival assays indicated the roles of PAL in regulating cell growth. (G) Live/dead cell staining indicated the roles of PAL in regulating cell death. Calcein AM (green) stained live cells, and PI (red) labeled dead cells. NS, not significant. * P < 0.05; ** P < 0.01. HCC, hepatocellular carcinoma; CCK-8, Cell Counting Kit-8; PAL, palmatine; PI, propidium iodide; AM, acetoxymethyl ester; DMSO, dimethyl sulfoxide.

Journal: Journal of Clinical and Translational Hepatology

Article Title: Palmatine Induces TNNT1 Ubiquitination and Degradation to Potentiate Ferroptosis Sensitivity in Hepatocellular Carcinoma

doi: 10.14218/JCTH.2026.00215

Figure Lengend Snippet: (A) Schematic workflow of alkaloid library screening for anticancer candidates. (B) The CCK-8 assay indicated the inhibitory effects of 269 alkaloid compounds on HCC cell viability. The top three candidate alkaloids with obvious anti-HCC activity were also displayed. (C-D) The time- and dose-dependent effects of PAL on HCC cells Huh7 and HepG2. (E) Changes in the viability of normal hepatic HHL5 cells after PAL treatment. (F) Clonogenic survival assays indicated the roles of PAL in regulating cell growth. (G) Live/dead cell staining indicated the roles of PAL in regulating cell death. Calcein AM (green) stained live cells, and PI (red) labeled dead cells. NS, not significant. * P < 0.05; ** P < 0.01. HCC, hepatocellular carcinoma; CCK-8, Cell Counting Kit-8; PAL, palmatine; PI, propidium iodide; AM, acetoxymethyl ester; DMSO, dimethyl sulfoxide.

Article Snippet: Cell death was quantified using the Calcein/PI Live/Dead Viability/Cytotoxicity Assay Kit (C2015M, Beyotime) following a previously described protocol.

Techniques: Library Screening, CCK-8 Assay, Activity Assay, Staining, Labeling, Cell Counting