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( A - D ) Pannexins were expressed in HEK293 cells and voltage clamp recordings were performed at –60 mV. Both the first round ( A and B ) and the second round ( C and D ) fractions are shown. Blue bars indicate application of each fraction. ( E ) Current densities from second-round fractions. The number of recordings from different cells for each experiment (biological replicates) is shown in parenthesis. Error bars represent SEM. ( F ) and ( G ) Whole-cell currents obtained with <t>vector-transfected</t> control cells. Application of fraction #12 from the first round and fraction #7 from the second round are shown. Figure 1—figure supplement 2—source data 1. Electrophysiology data used for .
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( A - D ) Pannexins were expressed in HEK293 cells and voltage clamp recordings were performed at –60 mV. Both the first round ( A and B ) and the second round ( C and D ) fractions are shown. Blue bars indicate application of each fraction. ( E ) Current densities from second-round fractions. The number of recordings from different cells for each experiment (biological replicates) is shown in parenthesis. Error bars represent SEM. ( F ) and ( G ) Whole-cell currents obtained with <t>vector-transfected</t> control cells. Application of fraction #12 from the first round and fraction #7 from the second round are shown. Figure 1—figure supplement 2—source data 1. Electrophysiology data used for .
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( A - D ) Pannexins were expressed in HEK293 cells and voltage clamp recordings were performed at –60 mV. Both the first round ( A and B ) and the second round ( C and D ) fractions are shown. Blue bars indicate application of each fraction. ( E ) Current densities from second-round fractions. The number of recordings from different cells for each experiment (biological replicates) is shown in parenthesis. Error bars represent SEM. ( F ) and ( G ) Whole-cell currents obtained with vector-transfected control cells. Application of fraction #12 from the first round and fraction #7 from the second round are shown. Figure 1—figure supplement 2—source data 1. Electrophysiology data used for .

Journal: eLife

Article Title: ATP-release pannexin channels are gated by lysophospholipids

doi: 10.7554/eLife.107067

Figure Lengend Snippet: ( A - D ) Pannexins were expressed in HEK293 cells and voltage clamp recordings were performed at –60 mV. Both the first round ( A and B ) and the second round ( C and D ) fractions are shown. Blue bars indicate application of each fraction. ( E ) Current densities from second-round fractions. The number of recordings from different cells for each experiment (biological replicates) is shown in parenthesis. Error bars represent SEM. ( F ) and ( G ) Whole-cell currents obtained with vector-transfected control cells. Application of fraction #12 from the first round and fraction #7 from the second round are shown. Figure 1—figure supplement 2—source data 1. Electrophysiology data used for .

Article Snippet: Transfected construct ( Homo-sapiens ) , PANX1 - shRNA #2 , BROAD Institute Genetic Perturbation Platform , TRCN0000154636 , Lentiviral construct to transfect and express the shRNA.

Techniques: Plasmid Preparation, Transfection, Control

Journal: eLife

Article Title: ATP-release pannexin channels are gated by lysophospholipids

doi: 10.7554/eLife.107067

Figure Lengend Snippet:

Article Snippet: Transfected construct ( Homo-sapiens ) , PANX1 - shRNA #2 , BROAD Institute Genetic Perturbation Platform , TRCN0000154636 , Lentiviral construct to transfect and express the shRNA.

Techniques: Recombinant, Synthesized, Expressing, Plasmid Preparation, Transfection, Construct, shRNA

Journal: eLife

Article Title: N6-methyladenosine in DNA promotes genome stability

doi: 10.7554/eLife.101626

Figure Lengend Snippet:

Article Snippet: Transfected construct ( Homo sapiens ) , Human: DLD-1 UNG KO Clone E7 + pCMV_Cry2_mCherry_UNG2_ DPIP , This paper , This paper , Pfizer, Inc.

Techniques: Transfection, Construct, Recombinant, Retroviral, Plasmid Preparation, Sequencing, Purification, MTS Assay, Saline, Cell Culture, Labeling, Mass Spectrometry, Blocking Assay, Electroporation, CRISPR, Gene Expression, TaqMan Assay, Multiplex sample analysis, Protease Inhibitor, Software, Genome Wide, Knock-Out, Immunofluorescence, Microscopy, Imaging, Western Blot