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Antibacterial evaluation of Mn‐ZIF‐8@GelMA hydrogels. Live/Dead staining images of (A) E. coli and (D) S. <t>mutans</t> cultured on different hydrogels ( n = 3 each group). Scale bar: 100 µm. Green indicates live bacteria, and red indicates dead bacteria, with the proportion of dead cells increasing as Mn‐ZIF‐8 concentration increased. Quantitative analysis of bacterial viability of (B) E. coli and (E) S. mutans in each group ( n = 3 each group). OD600 values of (C) E. coli and (F) S. mutans showing bacterial growth inhibition with increasing Mn‐ZIF‐8 content ( n = 3 each group). Error bars indicate the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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Antibacterial evaluation of Mn‐ZIF‐8@GelMA hydrogels. Live/Dead staining images of (A) E. coli and (D) S. <t>mutans</t> cultured on different hydrogels ( n = 3 each group). Scale bar: 100 µm. Green indicates live bacteria, and red indicates dead bacteria, with the proportion of dead cells increasing as Mn‐ZIF‐8 concentration increased. Quantitative analysis of bacterial viability of (B) E. coli and (E) S. mutans in each group ( n = 3 each group). OD600 values of (C) E. coli and (F) S. mutans showing bacterial growth inhibition with increasing Mn‐ZIF‐8 content ( n = 3 each group). Error bars indicate the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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Antibacterial evaluation of Mn‐ZIF‐8@GelMA hydrogels. Live/Dead staining images of (A) E. coli and (D) S. <t>mutans</t> cultured on different hydrogels ( n = 3 each group). Scale bar: 100 µm. Green indicates live bacteria, and red indicates dead bacteria, with the proportion of dead cells increasing as Mn‐ZIF‐8 concentration increased. Quantitative analysis of bacterial viability of (B) E. coli and (E) S. mutans in each group ( n = 3 each group). OD600 values of (C) E. coli and (F) S. mutans showing bacterial growth inhibition with increasing Mn‐ZIF‐8 content ( n = 3 each group). Error bars indicate the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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atcc 25175  (ATCC)
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Antibacterial evaluation of Mn‐ZIF‐8@GelMA hydrogels. Live/Dead staining images of (A) E. coli and (D) S. <t>mutans</t> cultured on different hydrogels ( n = 3 each group). Scale bar: 100 µm. Green indicates live bacteria, and red indicates dead bacteria, with the proportion of dead cells increasing as Mn‐ZIF‐8 concentration increased. Quantitative analysis of bacterial viability of (B) E. coli and (E) S. mutans in each group ( n = 3 each group). OD600 values of (C) E. coli and (F) S. mutans showing bacterial growth inhibition with increasing Mn‐ZIF‐8 content ( n = 3 each group). Error bars indicate the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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Antibacterial evaluation of Mn‐ZIF‐8@GelMA hydrogels. Live/Dead staining images of (A) E. coli and (D) S. <t>mutans</t> cultured on different hydrogels ( n = 3 each group). Scale bar: 100 µm. Green indicates live bacteria, and red indicates dead bacteria, with the proportion of dead cells increasing as Mn‐ZIF‐8 concentration increased. Quantitative analysis of bacterial viability of (B) E. coli and (E) S. mutans in each group ( n = 3 each group). OD600 values of (C) E. coli and (F) S. mutans showing bacterial growth inhibition with increasing Mn‐ZIF‐8 content ( n = 3 each group). Error bars indicate the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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streptococcus mutans ua159  (ATCC)
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Antibacterial evaluation of Mn‐ZIF‐8@GelMA hydrogels. Live/Dead staining images of (A) E. coli and (D) S. <t>mutans</t> cultured on different hydrogels ( n = 3 each group). Scale bar: 100 µm. Green indicates live bacteria, and red indicates dead bacteria, with the proportion of dead cells increasing as Mn‐ZIF‐8 concentration increased. Quantitative analysis of bacterial viability of (B) E. coli and (E) S. mutans in each group ( n = 3 each group). OD600 values of (C) E. coli and (F) S. mutans showing bacterial growth inhibition with increasing Mn‐ZIF‐8 content ( n = 3 each group). Error bars indicate the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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Antibacterial evaluation of Mn‐ZIF‐8@GelMA hydrogels. Live/Dead staining images of (A) E. coli and (D) S. mutans cultured on different hydrogels ( n = 3 each group). Scale bar: 100 µm. Green indicates live bacteria, and red indicates dead bacteria, with the proportion of dead cells increasing as Mn‐ZIF‐8 concentration increased. Quantitative analysis of bacterial viability of (B) E. coli and (E) S. mutans in each group ( n = 3 each group). OD600 values of (C) E. coli and (F) S. mutans showing bacterial growth inhibition with increasing Mn‐ZIF‐8 content ( n = 3 each group). Error bars indicate the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Advanced Science

Article Title: Mn/Zn Bimetallic MOF‐Integrated Hydrogel Alleviates Excessive Mitochondrial Fission in BMSCs to Promote Osteoporotic Jawbone Repair

doi: 10.1002/advs.78268

Figure Lengend Snippet: Antibacterial evaluation of Mn‐ZIF‐8@GelMA hydrogels. Live/Dead staining images of (A) E. coli and (D) S. mutans cultured on different hydrogels ( n = 3 each group). Scale bar: 100 µm. Green indicates live bacteria, and red indicates dead bacteria, with the proportion of dead cells increasing as Mn‐ZIF‐8 concentration increased. Quantitative analysis of bacterial viability of (B) E. coli and (E) S. mutans in each group ( n = 3 each group). OD600 values of (C) E. coli and (F) S. mutans showing bacterial growth inhibition with increasing Mn‐ZIF‐8 content ( n = 3 each group). Error bars indicate the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Streptococcus mutans ( S. mutans , ATCC 25175) was cultured in brain heart infusion (BHI) broth, and Escherichia coli (E. coli , ATCC 25922) was cultured in Luria‐Bertani (LB) medium at 37°C for 48 h and subcultured for subsequent experiments.

Techniques: Staining, Cell Culture, Bacteria, Concentration Assay, Analysis, Inhibition