Journal: Journal of Biological Chemistry

Article Title: Hypoxia-induced Regulation of MAPK Phosphatase-1 as Identified by Subtractive Suppression Hybridization and cDNA Microarray Analysis

doi: 10.1074/jbc.m103346200

Figure Lengend Snippet: FIG. 1. MKP-1 mRNA levels are up- regulated by hypoxia in microarray analysis. PC12 cells were exposed to nor- moxia or hypoxia (1% O2) for 6 h, and total RNA was isolated as described under “Ex- perimental Procedures.” A, comparison of control versus control gene expression pattern. Cy3- and Cy5-labeled probes were each generated from RNA derived from cells maintained in normoxia. Probes were hybridized to arrays as de- scribed under “Experimental Proce- dures.” Background-subtracted median pixel intensities are plotted from a repre- sentative experiment. Each symbol repre- sents one spot on the array. B, compari- son of control versus hypoxia gene expression pattern. The Cy5-labeled probe was generated from RNA derived from cells maintained in normoxia, whereas the Cy3-labeled probe was gen- erated from RNA derived from cells ex- posed to hypoxia. The data shown are rep- resentative of results obtained in three separate hybridization experiments.

Article Snippet: Microarray Data Analysis—Slides were scanned with a GenePix 4000A scanner (Axon Instruments, Inc., Foster City, CA) at 532 nm (Cy3) and 635 nm (Cy5) simultaneously.

Techniques: Microarray, Isolation, Comparison, Control, Gene Expression, Labeling, Generated, Derivative Assay, Hybridization