Journal: PLOS One

Article Title: Metabolomics and glucose tolerance in pregnancy and postpartum: The PONCH study

doi: 10.1371/journal.pone.0335708

Figure Lengend Snippet: Selected clinical measurements (A) and metabolites (B) are compared between NW (n = 33), OB (n = 32), GDM-NW (n = 15) and GDM-OB (n = 16) women. The boxes represent the 25 th to 75 th percentiles with Tukey whiskers and the line indicating median. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 for group comparisons using Kruskal-Wallis analysis and Mann-Whitney-U statistics adjusted for multiple comparisons. Observed power for Kruskal-Wallis analysis was ≥ 0.8 for all variables except one (Valine, power = 0.4) C) Overview of significant differences. NW, women of normal weight; OB, women with obesity; GDM-NW, women with GDM and of normal weight; GDM-OB, women with GDM and obesity; LepR, soluble leptin receptor; HOMA-IR, homeostatic model assessment of insulin resistance; BCAA, branched chained amino acids.

Article Snippet: Leptin (Human Leptin Quantikine, R&D Systems, Minneapolis, MN; interassay coefficient of variation, 8.0% at 9 μg/l), the soluble leptin receptor (LepR) (Human Leptin R Quantikine, R&D Systems) and adiponectin (Human Adiponectin ELISA kit, Millipore, Billerica, MA; interassay coefficient of variation, 7.0% at 10.5 mg/l) were analysed simultaneously for all samples using ELISA.

Techniques: MANN-WHITNEY

Journal: bioRxiv

Article Title: Quantitative Multicolored Deep Imaging of Human Bones Reveals a Composite Osteo-Sinusoidal Niche for Mesenchymal Stromal Cells

doi: 10.1101/2025.10.07.680053

Figure Lengend Snippet: a,b , Colocalization of LEPR protein (antibody staining, pseudocoloured yellow) and CXCL12 mRNA (HCR RNA-FISH, red) in representative 2D optical sections from ( a ) a 3D light-sheet scan and ( b ) a confocal scan of tissue-cleared human bone. Left panels show LEPR omitted; right panels include all channels. Nuclei (YOPRO, green) and bone matrix (autofluorescence at 405 nm, cyan) were visualized in ( a ) but not ( b ) due to sample bleaching. c , Confocal image of a 6-μm thick section from decalcified human bone stained for LEPR (green), CXCL12 mRNA (red), and counterstained with DAPI (blue). Single and merged channels are shown. d–f , Nuclear staining of aged human bone with YOPRO1 (green). d, Raw YOPRO+ signals in a light-sheet scan. e, YOPRO+ nuclei overlaid with cell positions detected by the Imaris Spot function. f, Optical sections showing overlay of YOPRO+ nuclei with segmented cell masks (grey circles). g–l , Detection of nucleated CXCL12+ cells in aged bone. g–i, Representative 2D optical sections from a 3D light-sheet scan showing CXCL12 mRNA (HCR, red), CD31+ vessels (yellow), and YOPRO1+ nuclei (green). CXCL12 signal is omitted in g and present in h ; i , colocalized CXCL12+YOPRO+ cells detected by Imaris Spot function are marked by grey circles. j–l , 3D projections of the same region as a raw scan ( j ) and detected double-positive cells at low ( k ) and high ( l ) magnification, with double-positive cells represented as green spheres. j , CD31 and YOPRO channels only; k,l , all channels including CXCL12. All images are cropped from large tissue volumes (∼2 × 2 × 2 mm) scanned by light-sheet microscopy.

Article Snippet: Following HCR, tissue sections were blocked using the same staining buffer as used in DeepBone, then incubated with primary antibodies against LEPR (conjugated to AlexaFluor488; #MAB867, R&D Systems) or Ki67 (conjugated to AlexaFluor546; SolA15, Thermofisher).

Techniques: Staining, Microscopy

Journal: iScience

Article Title: Immunometabolic adaptation in monocytes underpins functional changes during pregnancy

doi: 10.1016/j.isci.2024.109779

Figure Lengend Snippet:

Article Snippet: CD295/LEPR human antibody (clone REA361) , Miltenyi , Cat#130-125-203; RRID: AB_2889537.

Techniques: Recombinant, Adhesive, Sequencing, Modification, DC Protein Assay, Staining, Imaging, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Gene Expression, Software, Saline