Journal: eLife

Article Title: In vivo virus-free lineage tracing and live imaging reveal that NeuroD1 does not reprogram microglia into neurons

doi: 10.7554/eLife.112083

Figure Lengend Snippet: ( A ) Experimental timeline. ( B ) Representative confocal images at D4, D10, D30, and D45 showing that GFP is not co-labeled with TUJ1 or MAP2 in the brain. ( C ) Quantification of TUJ1 + GFP + double-positive cells among TUJ1 + cells at D4, D10, D30, and D45. N = 4 or 5 mice for each group. ( D ) Quantification of MAP2 + GFP + double-positive cells among MAP2 + cells at D4, D10, D30, and D45. N = 4 or 5 mice for each group. ( E ) Relative gene expression level at D18. N = 4 mice for each group. Data are presented as mean ± SD. Each data point represents one mouse.

Article Snippet: The cell pellet was then resuspended in 90 μl of 0.5% BSA in DPBS and incubated with 10 μl of anti-CD11b magnetic beads (Miltenyi, Cat#: 130-097-142) for 30 min at 4°C.

Techniques: Confocal, Labeling, Gene Expression, Magnetic Cell Separation, Real-time Polymerase Chain Reaction

Journal: eLife

Article Title: In vivo virus-free lineage tracing and live imaging reveal that NeuroD1 does not reprogram microglia into neurons

doi: 10.7554/eLife.112083

Figure Lengend Snippet: Using qPCR verify the relative expression of Neurod1 .

Article Snippet: The cell pellet was then resuspended in 90 μl of 0.5% BSA in DPBS and incubated with 10 μl of anti-CD11b magnetic beads (Miltenyi, Cat#: 130-097-142) for 30 min at 4°C.

Techniques: Expressing, Gene Expression

Journal: Emerging Microbes & Infections

Article Title: A novel CXCL8(3–72) K11R/G31P–BPI 233 hybrid protein modulates neutrophil-driven inflammation and protects against carbapenem-resistant Acinetobacter baumannii infection

doi: 10.1080/22221751.2026.2741787

Figure Lengend Snippet: G31P–BPI 233 limits neutrophil-associated inflammation during CRAB pneumonia. Mice were intranasally infected with A. baumannii ATCC BAA-1605 and treated with saline, G31P, BPI 233 , an equimolar G31P + BPI 233 mixture, G31P–BPI 233 , or colistin (n = 5 per group). Uninfected mice served as normal controls (n = 3). Samples were collected 24 h after infection. (A–D) Representative flow-cytometry plots and quantification of CD45⁺CD11b⁺Ly6G⁺ neutrophils among lung (A, B) and peripheral blood (C, D) leukocytes. (E, F) Pulmonary levels of MPO and extracellular dsDNA. Component doses were molar-matched to the corresponding fusion-protein dose. Data are presented as mean ± SEM. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Human neutrophils were identified as CD45+CD16+CD66b+ cells using the following antibodies: anti-human CD45 (BioLegend, Cat# 368526), anti-human CD16 (BioLegend, Cat# 980102), and anti-human CD66b (BioLegend, Cat# 305108) [ ], and mouse BM neutrophils as CD45+Ly6G+CD11b+ cells using the following antibodies: anti-CD11b (Miltenyi Biotec, Cat# 130-113-805), anti-Ly6G (Miltenyi Biotec, Cat# 130-119-126), and anti-CD45 (Miltenyi Biotec, Cat# 130-110-803) [ ].

Techniques: Infection, Saline, Flow Cytometry