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Characterization of mechanically isolated hormone-producing ovarian cells. (A) Hormone-producing ovarian cells are isolated by microdissection from the E 2 -primed ovary of 3-week-old female rats. (B) Morphology of granulosa cells (GCs) and theca cells (TCs) during a 72-h culture. Scale bars, 100 μm. (C) Fluorescence images of the staining of GC and TC with <t>anti-CYP19</t> granulosa cell marker (green), anti-CYP17A1 theca cell marker (green), and DAPI (blue) nuclear counterstain. Scale bars, 20 μm. (D) The quantitative analysis of cell purity after staining of anti-CYP19 granulosa cell marker and anti-CYP17A1 theca cell marker, respectively, using flow cytometry.
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Characterization of mechanically isolated hormone-producing ovarian cells. (A) Hormone-producing ovarian cells are isolated by microdissection from the E 2 -primed ovary of 3-week-old female rats. (B) Morphology of granulosa cells (GCs) and theca cells (TCs) during a 72-h culture. Scale bars, 100 μm. (C) Fluorescence images of the staining of GC and TC with anti-CYP19 granulosa cell marker (green), anti-CYP17A1 theca cell marker (green), and DAPI (blue) nuclear counterstain. Scale bars, 20 μm. (D) The quantitative analysis of cell purity after staining of anti-CYP19 granulosa cell marker and anti-CYP17A1 theca cell marker, respectively, using flow cytometry.

Journal: Biomaterials Research

Article Title: Injectable Biomimetic Hydrogel Constructs for Cell-Based Menopausal Hormone Therapy with Reduced Breast Cancer Potential

doi: 10.34133/bmr.0054

Figure Lengend Snippet: Characterization of mechanically isolated hormone-producing ovarian cells. (A) Hormone-producing ovarian cells are isolated by microdissection from the E 2 -primed ovary of 3-week-old female rats. (B) Morphology of granulosa cells (GCs) and theca cells (TCs) during a 72-h culture. Scale bars, 100 μm. (C) Fluorescence images of the staining of GC and TC with anti-CYP19 granulosa cell marker (green), anti-CYP17A1 theca cell marker (green), and DAPI (blue) nuclear counterstain. Scale bars, 20 μm. (D) The quantitative analysis of cell purity after staining of anti-CYP19 granulosa cell marker and anti-CYP17A1 theca cell marker, respectively, using flow cytometry.

Article Snippet: The primary antibodies used in this study are anti-CYP19 (BS-1292R, Bioss, Woburn, MA, USA) and anti-CYP17A1 (BS-54306R, Bioss).

Techniques: Isolation, Laser Capture Microdissection, Fluorescence, Staining, Marker, Flow Cytometry

Information on the antibodies used in this study.

Journal: Animals : an Open Access Journal from MDPI

Article Title: NTRK2 Promotes Sheep Granulosa Cells Proliferation and Reproductive Hormone Secretion and Activates the PI3K/AKT Pathway

doi: 10.3390/ani14101465

Figure Lengend Snippet: Information on the antibodies used in this study.

Article Snippet: bs-1292R , anti-Aromatase , 1:1000 , Bioss, Beijing, China.

Techniques: