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null adenovirus  (Vector Biolabs)
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Vector Biolabs null adenovirus
A) AML12 cells transduced with ATGL or GFP control <t>adenoviruses</t> were treated with 30 μM etoposide for the indicated times and assayed for γH2Ax. B) Quantification of A. *p<0.05, **p<0.01. C) AML12 cells transduced with ATGL or GFP control adenoviruses were irradiated (10 Gy) and harvested at the indicated times post-IR. D) Quantification of C. **p<0.01. E) Primary MEFs were isolated from WT or ATGL knock-in (AKI) mice, followed by etoposide incubation for 6 hours. Immunofluorescence was performed for γH2Ax, and cells were imaged using the Agilent Gen5 Cytation. Representative γH2Ax images and foci distribution are shown. F) Cells were treated with DGAT inhibitors for 24 hours, followed by ATGL adenoviral transduction, followed by 24 hours of etoposide treatment. Western blotting for γH2Ax, β-actin, and ATGL. G) AML12 cells were treated as in F. The Comet assay was performed on cells after 24 hours of etoposide. Comets were quantified with OpenComet software. A minimum of 180 comets were quantified per condition. Statistics: one-way ANOVA with Tukey’s post hoc test. ****p<0.0001. H) γH2Ax results and quantification from tissues harvested from E2A Cre-ATGL (AKI) mice irradiated with 7.5 Gy + 4-hour recovery. Mice were of mixed sexes, n=4 mice. P-values from one-way ANOVA are displayed
Null Adenovirus, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ad cmv  (Vector Biolabs)
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A) AML12 cells transduced with ATGL or GFP control <t>adenoviruses</t> were treated with 30 μM etoposide for the indicated times and assayed for γH2Ax. B) Quantification of A. *p<0.05, **p<0.01. C) AML12 cells transduced with ATGL or GFP control adenoviruses were irradiated (10 Gy) and harvested at the indicated times post-IR. D) Quantification of C. **p<0.01. E) Primary MEFs were isolated from WT or ATGL knock-in (AKI) mice, followed by etoposide incubation for 6 hours. Immunofluorescence was performed for γH2Ax, and cells were imaged using the Agilent Gen5 Cytation. Representative γH2Ax images and foci distribution are shown. F) Cells were treated with DGAT inhibitors for 24 hours, followed by ATGL adenoviral transduction, followed by 24 hours of etoposide treatment. Western blotting for γH2Ax, β-actin, and ATGL. G) AML12 cells were treated as in F. The Comet assay was performed on cells after 24 hours of etoposide. Comets were quantified with OpenComet software. A minimum of 180 comets were quantified per condition. Statistics: one-way ANOVA with Tukey’s post hoc test. ****p<0.0001. H) γH2Ax results and quantification from tissues harvested from E2A Cre-ATGL (AKI) mice irradiated with 7.5 Gy + 4-hour recovery. Mice were of mixed sexes, n=4 mice. P-values from one-way ANOVA are displayed
Ad Cmv, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cmv promoter  (Vector Biolabs)
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Vector Biolabs cmv promoter
A) AML12 cells transduced with ATGL or GFP control <t>adenoviruses</t> were treated with 30 μM etoposide for the indicated times and assayed for γH2Ax. B) Quantification of A. *p<0.05, **p<0.01. C) AML12 cells transduced with ATGL or GFP control adenoviruses were irradiated (10 Gy) and harvested at the indicated times post-IR. D) Quantification of C. **p<0.01. E) Primary MEFs were isolated from WT or ATGL knock-in (AKI) mice, followed by etoposide incubation for 6 hours. Immunofluorescence was performed for γH2Ax, and cells were imaged using the Agilent Gen5 Cytation. Representative γH2Ax images and foci distribution are shown. F) Cells were treated with DGAT inhibitors for 24 hours, followed by ATGL adenoviral transduction, followed by 24 hours of etoposide treatment. Western blotting for γH2Ax, β-actin, and ATGL. G) AML12 cells were treated as in F. The Comet assay was performed on cells after 24 hours of etoposide. Comets were quantified with OpenComet software. A minimum of 180 comets were quantified per condition. Statistics: one-way ANOVA with Tukey’s post hoc test. ****p<0.0001. H) γH2Ax results and quantification from tissues harvested from E2A Cre-ATGL (AKI) mice irradiated with 7.5 Gy + 4-hour recovery. Mice were of mixed sexes, n=4 mice. P-values from one-way ANOVA are displayed
Cmv Promoter, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ad cmv null  (Vector Biolabs)
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Vector Biolabs ad cmv null
A) AML12 cells transduced with ATGL or GFP control <t>adenoviruses</t> were treated with 30 μM etoposide for the indicated times and assayed for γH2Ax. B) Quantification of A. *p<0.05, **p<0.01. C) AML12 cells transduced with ATGL or GFP control adenoviruses were irradiated (10 Gy) and harvested at the indicated times post-IR. D) Quantification of C. **p<0.01. E) Primary MEFs were isolated from WT or ATGL knock-in (AKI) mice, followed by etoposide incubation for 6 hours. Immunofluorescence was performed for γH2Ax, and cells were imaged using the Agilent Gen5 Cytation. Representative γH2Ax images and foci distribution are shown. F) Cells were treated with DGAT inhibitors for 24 hours, followed by ATGL adenoviral transduction, followed by 24 hours of etoposide treatment. Western blotting for γH2Ax, β-actin, and ATGL. G) AML12 cells were treated as in F. The Comet assay was performed on cells after 24 hours of etoposide. Comets were quantified with OpenComet software. A minimum of 180 comets were quantified per condition. Statistics: one-way ANOVA with Tukey’s post hoc test. ****p<0.0001. H) γH2Ax results and quantification from tissues harvested from E2A Cre-ATGL (AKI) mice irradiated with 7.5 Gy + 4-hour recovery. Mice were of mixed sexes, n=4 mice. P-values from one-way ANOVA are displayed
Ad Cmv Null, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ad-cmv-null  (Vector Biolabs)
96
Vector Biolabs ad-cmv-null
A) AML12 cells transduced with ATGL or GFP control <t>adenoviruses</t> were treated with 30 μM etoposide for the indicated times and assayed for γH2Ax. B) Quantification of A. *p<0.05, **p<0.01. C) AML12 cells transduced with ATGL or GFP control adenoviruses were irradiated (10 Gy) and harvested at the indicated times post-IR. D) Quantification of C. **p<0.01. E) Primary MEFs were isolated from WT or ATGL knock-in (AKI) mice, followed by etoposide incubation for 6 hours. Immunofluorescence was performed for γH2Ax, and cells were imaged using the Agilent Gen5 Cytation. Representative γH2Ax images and foci distribution are shown. F) Cells were treated with DGAT inhibitors for 24 hours, followed by ATGL adenoviral transduction, followed by 24 hours of etoposide treatment. Western blotting for γH2Ax, β-actin, and ATGL. G) AML12 cells were treated as in F. The Comet assay was performed on cells after 24 hours of etoposide. Comets were quantified with OpenComet software. A minimum of 180 comets were quantified per condition. Statistics: one-way ANOVA with Tukey’s post hoc test. ****p<0.0001. H) γH2Ax results and quantification from tissues harvested from E2A Cre-ATGL (AKI) mice irradiated with 7.5 Gy + 4-hour recovery. Mice were of mixed sexes, n=4 mice. P-values from one-way ANOVA are displayed
Ad Cmv Null, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adenovirus mediated adv control  (Vector Biolabs)
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Vector Biolabs adenovirus mediated adv control
A) AML12 cells transduced with ATGL or GFP control <t>adenoviruses</t> were treated with 30 μM etoposide for the indicated times and assayed for γH2Ax. B) Quantification of A. *p<0.05, **p<0.01. C) AML12 cells transduced with ATGL or GFP control adenoviruses were irradiated (10 Gy) and harvested at the indicated times post-IR. D) Quantification of C. **p<0.01. E) Primary MEFs were isolated from WT or ATGL knock-in (AKI) mice, followed by etoposide incubation for 6 hours. Immunofluorescence was performed for γH2Ax, and cells were imaged using the Agilent Gen5 Cytation. Representative γH2Ax images and foci distribution are shown. F) Cells were treated with DGAT inhibitors for 24 hours, followed by ATGL adenoviral transduction, followed by 24 hours of etoposide treatment. Western blotting for γH2Ax, β-actin, and ATGL. G) AML12 cells were treated as in F. The Comet assay was performed on cells after 24 hours of etoposide. Comets were quantified with OpenComet software. A minimum of 180 comets were quantified per condition. Statistics: one-way ANOVA with Tukey’s post hoc test. ****p<0.0001. H) γH2Ax results and quantification from tissues harvested from E2A Cre-ATGL (AKI) mice irradiated with 7.5 Gy + 4-hour recovery. Mice were of mixed sexes, n=4 mice. P-values from one-way ANOVA are displayed
Adenovirus Mediated Adv Control, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adenovirus  (Vector Biolabs)
96
Vector Biolabs adenovirus
A) AML12 cells transduced with ATGL or GFP control <t>adenoviruses</t> were treated with 30 μM etoposide for the indicated times and assayed for γH2Ax. B) Quantification of A. *p<0.05, **p<0.01. C) AML12 cells transduced with ATGL or GFP control adenoviruses were irradiated (10 Gy) and harvested at the indicated times post-IR. D) Quantification of C. **p<0.01. E) Primary MEFs were isolated from WT or ATGL knock-in (AKI) mice, followed by etoposide incubation for 6 hours. Immunofluorescence was performed for γH2Ax, and cells were imaged using the Agilent Gen5 Cytation. Representative γH2Ax images and foci distribution are shown. F) Cells were treated with DGAT inhibitors for 24 hours, followed by ATGL adenoviral transduction, followed by 24 hours of etoposide treatment. Western blotting for γH2Ax, β-actin, and ATGL. G) AML12 cells were treated as in F. The Comet assay was performed on cells after 24 hours of etoposide. Comets were quantified with OpenComet software. A minimum of 180 comets were quantified per condition. Statistics: one-way ANOVA with Tukey’s post hoc test. ****p<0.0001. H) γH2Ax results and quantification from tissues harvested from E2A Cre-ATGL (AKI) mice irradiated with 7.5 Gy + 4-hour recovery. Mice were of mixed sexes, n=4 mice. P-values from one-way ANOVA are displayed
Adenovirus, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A) AML12 cells transduced with ATGL or GFP control adenoviruses were treated with 30 μM etoposide for the indicated times and assayed for γH2Ax. B) Quantification of A. *p<0.05, **p<0.01. C) AML12 cells transduced with ATGL or GFP control adenoviruses were irradiated (10 Gy) and harvested at the indicated times post-IR. D) Quantification of C. **p<0.01. E) Primary MEFs were isolated from WT or ATGL knock-in (AKI) mice, followed by etoposide incubation for 6 hours. Immunofluorescence was performed for γH2Ax, and cells were imaged using the Agilent Gen5 Cytation. Representative γH2Ax images and foci distribution are shown. F) Cells were treated with DGAT inhibitors for 24 hours, followed by ATGL adenoviral transduction, followed by 24 hours of etoposide treatment. Western blotting for γH2Ax, β-actin, and ATGL. G) AML12 cells were treated as in F. The Comet assay was performed on cells after 24 hours of etoposide. Comets were quantified with OpenComet software. A minimum of 180 comets were quantified per condition. Statistics: one-way ANOVA with Tukey’s post hoc test. ****p<0.0001. H) γH2Ax results and quantification from tissues harvested from E2A Cre-ATGL (AKI) mice irradiated with 7.5 Gy + 4-hour recovery. Mice were of mixed sexes, n=4 mice. P-values from one-way ANOVA are displayed

Journal: bioRxiv

Article Title: ATGL-catalyzed lipid catabolism promotes DNA repair

doi: 10.64898/2026.04.03.716381

Figure Lengend Snippet: A) AML12 cells transduced with ATGL or GFP control adenoviruses were treated with 30 μM etoposide for the indicated times and assayed for γH2Ax. B) Quantification of A. *p<0.05, **p<0.01. C) AML12 cells transduced with ATGL or GFP control adenoviruses were irradiated (10 Gy) and harvested at the indicated times post-IR. D) Quantification of C. **p<0.01. E) Primary MEFs were isolated from WT or ATGL knock-in (AKI) mice, followed by etoposide incubation for 6 hours. Immunofluorescence was performed for γH2Ax, and cells were imaged using the Agilent Gen5 Cytation. Representative γH2Ax images and foci distribution are shown. F) Cells were treated with DGAT inhibitors for 24 hours, followed by ATGL adenoviral transduction, followed by 24 hours of etoposide treatment. Western blotting for γH2Ax, β-actin, and ATGL. G) AML12 cells were treated as in F. The Comet assay was performed on cells after 24 hours of etoposide. Comets were quantified with OpenComet software. A minimum of 180 comets were quantified per condition. Statistics: one-way ANOVA with Tukey’s post hoc test. ****p<0.0001. H) γH2Ax results and quantification from tissues harvested from E2A Cre-ATGL (AKI) mice irradiated with 7.5 Gy + 4-hour recovery. Mice were of mixed sexes, n=4 mice. P-values from one-way ANOVA are displayed

Article Snippet: For adenoviral transductions, ATGL (Lot: 20160819, mouse ATGL) was obtained from VectorBiolabs, TrackGFP (adGFP) was obtained from UNC Gene Therapy Center, and Null adenovirus was obtained from VectorBiolabs (#1300).

Techniques: Transduction, Control, Irradiation, Isolation, Knock-In, Incubation, Immunofluorescence, Western Blot, Single Cell Gel Electrophoresis, Software